Calderwood, and Jianlin Gong - Isolation of heat shock protein complexes / Fernando D. Gabai - Immunohistochemistry of human Hsp60 in health and disease : from autoimmunity to cancer / Francesco Cappello - Preparation of a heat-shock protein 70-based vaccine from DC-tumor fusion cells / Desheng Weng, Stuart K. Houry - Hsp70 : anti-apoptotic and tumorigenic protein / Anne-Laure Rérole, Gaëtan Jego, and Carmen Garrido - Determination of cell survival or death / Alexander E. Dempsey - Quantitation of heat-shock proteins in clinical samples using mass spectrometry / Punit Kaur and Alexzander Asea - Bioinformatic approach to identify chaperone pathway relationship from large-scale interaction networks / Yunchen Gong, Zhaolei Zhang, and Walid A.
Williams - Analysis of heat-shock protein localisation using flow cytometry / Nina C.
Nagaraja, and Alexzander Asea - Quantification of Hsp27 and Hsp70 molecular chaperone activities / Jessica Gobbo - Measuring Hsp72 (HSPA1A) by indirect sandwich ELISA / H.
Mayer, and Bernd Bukau - Reconstitution of CHIP E3 ubiquitin ligase activity / Hong Yu Ren - Structure-functions of HspB1 (Hsp27) / André-Patrick Arrigo - Combined lentiviral and RNAi technologies for the delivery and permanent silencing of the Hsp25 gene/ Punit Kaur, Ganachari M. Mandal - Nucleotide exchange factors for Hsp70 chaperones / Heike Rampelt, Matthias P. Johnson - Detecting Hsp90 phosphorylation / Mehdi Mollapour and Len Neckers - Role of molecular chaperones in biogenesis of the protein kinome / Atin K. Bolon - The role of p23, hop, immunophilins, and other co-chaperones in regulating Hsp90 function / Marc B. Targeted deletion of Hsf1, 2, and 4 genes in mice/ Xiongie Jin - The role of heat shock factors in stress-induced transcription / Yue Zhang - Hsp90 and client protein maturation / Natalie Wayne, Parul Mishra, and Daniel N. The relationship between the biophysical properties of biological macromolecules and their roles as molecular machines is emphasized throughout and illustrated with three examples DNA helicases, rotary motor ATPases, and myosin.The concluding chapter discusses future prospects in X-ray and neutron scattering, mass spectrometry, and pharmaceutical development. Subsequent chapters written by experts present, with examples, the major experimental methods: optical spectroscopy, X-ray and neutron diffraction and scattering, nuclear magnetic resonance, electron paramagnetic resonance, mass spectrometry, and single molecule methods. The book begins with an overview of the development of molecular biophysics and a brief survey of structural, physical, and chemical principles. The scope of this volume is appropriate for advanced undergraduate and graduate courses in biophysics and biophysical chemistry. It is designed to enable non-specialists -both students and professionals in other fields - to understand how these approaches can be used across the biosciences and in medicine, agriculture, biotechnology, pharmaceutical development and other fields.
HELIO RIPIT SERIES
This volume of the series Biophysics for the Life Sciences focuses on the conceptual framework and major research tools of contemporary molecular biophysics.
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